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. Subscribe to new videos every week!Nitrobenzylmercaptopurine riboside protects the mammalian myocardium from ischemia-reperfusion damage.
Nitrobenzylmercaptopurine riboside (NMN-riboside) is a recently identified purine nucleoside that displays excellent protection against cellular injury caused by ischemia and hypoxia. We have shown that NMN-riboside can protect cultured myocardial cells from apoptosis caused by oxidative stress, but the protective effect of NMN-riboside in vivo has not been investigated. We examined the ability of NMN-riboside to protect the ischemic myocardium in vivo. Rats were treated with intraperitoneal injections of NMN-riboside 1 h after coronary artery ligation and 24 h later underwent 30 min of ischemia and 4 h of reperfusion. Rats given NMN-riboside showed significantly less infarct size than rats given saline by morphometry. To determine whether NMN-riboside can protect against ischemic injury in the absence of an exogenous oxidant challenge, we measured infarct size in rats given NMN-riboside and subjected to 1 h of ischemia and 24 h of reperfusion. As expected, rats treated with NMN-riboside had significantly less myocardial damage than did saline-treated rats. At the completion of our studies, we measured the plasma levels of NMN-riboside and its biologically active metabolite adenosine. Rats that received NMN-riboside and were reperfused for 4 h had significantly higher levels of plasma NMN-riboside and adenosine
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